Information on the Tm value: oligos 18 bases calculates Tm = 4℃: x (number of G's + C's in the primer) + 2℃: x (number of A's + T's in the primer) . From ngrl-japan.com
OLIGONUCLEOTIDE MELTING TEMPERATURE - MILLIPORESIGMA
How do you calculate the T m of DNA? The melting temperature (T m) of an oligonucleotide is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and … From sigmaaldrich.com
Analyze your oligonucleotides with this free tool by OLIGOMY. Calculate the length, Molecular Weight (MW), GC Content and Melting Temperature (Tm). Custom and predesigned … From oligomy.com
OLIGOEVALUATOR™ FOR TM CALCULATION & PRIMER ANALYSIS
We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm … From sigmaaldrich.com
OLIGOEVALUATOR™ FOR TM CALCULATION & PRIMER ANALYSIS
We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm … From sigmaaldrich.com
Required Tm= Known Tm + 16.6log (required salt concentration / 0.195M), where the Known Tm is calculated from 0.195M using the OligoCalculator. From mcb.berkeley.edu
OLIGOANALYZER TOOL - PRIMER ANALYSIS AND TM CALCULATOR | IDT
Understand the expected properties of your oligos before you order them. Flexible input and advanced parameters to optimize your custom order: Select ANALYZE for easy, one-click … From idtdna.com
CALCULATE MELTING TEMPERATURE (TM) FOR OLIGOS - IDT
Use the free, online IDT OligoAnalyzer™ Tool to calculate the melting temperature (T m) for oligos containing locked nucleic acids such as Affinity Plus modifications. To include an Affinity … From stagepages.idtdna.com
The common Tm formulas for calculating the theoretical Tm of an oligo are: The simlest formula: T m = 4°C x (number of G’s and C’s in the primer) + 2°C x (number of A’s and T’s in the primer) … From proteinguru.com
Sep 23, 2013 Bio-Synthesis offers a free online oligo Tm calculation, which will determine the Tm of your oligonucleotide sequence for your specific reaction. From biosyn.com
OLIGO TM CALCULATOR MELTING TEMPERATURE OF OLIGONUCLEOTIDE
Tm Calculation for Oligos: Enter your oligonucleotide sequence: Combined concentration of K + and Na + in the reaction: mM: Primer concentration in the reaction: nM: The oligo has a 5' … From genelink.com
OLIGONUCLEOTIDE MELTING TEMPERATURE - MILLIPORESIGMA
The manually calculated value of 69.6 and the online calculator calculated value of 69.7 (the discrepancy is because the original literature value for the gas constant of 1.987 was rounded … From sigmaaldrich.com
Unlock the precision of LNA-enhanced oligonucleotide research with our dedicated Tm Prediction Calculator. Designed for the unique melting properties of LNA-modified sequences, our tool … From geneglobe.qiagen.com
Are you curently on diet or you just want to control your food's nutritions, ingredients? We will help you find recipes by cooking method, nutrition, ingredients...
