Carboxyfluorescein Succinimidyl Ester Food

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CFDA-SE | CELL PROLIFERATION AND TRACKING DYE - STEMCELL
cfda-se-cell-proliferation-and-tracking-dye-stemcell image
Web CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, …
From stemcell.com


5-CARBOXYFLUORESCEIN N -SUCCINIMIDYL ESTER
5-carboxyfluorescein-n-succinimidyl-ester image
Web 5-Carboxyfluorescein N-succinimidyl ester BioReagent, suitable for fluorescence, ≥90% (HPLC) Empirical Formula (Hill Notation): C25H15NO9 CAS Number: 92557-80-7 Molecular Weight: 473.39 Beilstein: 7865828 …
From sigmaaldrich.com


THE USE OF CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL …
Web Oct 12, 2010 PMC3185625 DOI: 10.3791/2259 Abstract Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division. CFSE covalently labels long-lived intracellular molecules with the fluorescent …
From pubmed.ncbi.nlm.nih.gov
Author Benjamin J. C. Quah, Christopher R. Parish
Publish Year 2010


CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER FLUORESCENT DYE …
Web Our objective was to study the properties of the carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and the methodology of cell labeling using CFDA-SE fluorescent dye. First, we analyzed the kinetics of CFDA-SE fluorescent dye intensity …
From pubmed.ncbi.nlm.nih.gov


CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER - AN OVERVIEW ...
Web Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled UC-MSCs have been shown to home to kidney, lung, liver, and spleen tissues in MRL/lpr lupus-prone mice with the highest levels being found at 24 h after infusion and remaining detectable for a week …
From sciencedirect.com


CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER - AN OVERVIEW
Web To address the issue of which cells proliferate in the MLR, the carboxyfluorescein succinimidyl ester (CFSE)-MLR was developed. In this assay, the responder (recipient) cells are labeled with CFSE that irreversibly binds to intracellular and cell-surface …
From sciencedirect.com


USING CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER TO MONITOR ...
Web Jun 1, 2015 Glyoxal (GO), an intracellular reactive oxoaldehyde that is one of the most potent glycation agents, readily reacts with amines present on proteins to produce the lysine-derived adduct carboxymethyllys … Using carboxyfluorescein diacetate …
From pubmed.ncbi.nlm.nih.gov


CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER FLUORESCENT DYE …
Web Jul 1, 2005 Carboxyfluorescein succinimidyl ester (CFSE) is a non-fluorescent dye that diffuses across cell membranes to be cleaved within the cell by intracellular esterases. The cleaved CFSE is highly ...
From researchgate.net


6-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER | C25H15NO9
Web 6-Carboxyfluorescein N-succinimidyl ester | C25H15NO9 | CID 2762614 - structure, chemical names, physical and chemical properties, classification, patents, literature ...
From pubchem.ncbi.nlm.nih.gov


CFSE | 99.85%(HPLC) | IN STOCK | DYES CHEMICAL
Web Carboxyfluorescein succinimidyl ester (CFSE, 5 (6)-Carboxyfluorescein diacetate succinimidyl ester, CFDA-SE, 5 (6)-CFDA N-succinmidyl ester) is a fluorescent cell staining dye. CFSE is frequently used in cell proliferation assay and motility assays.
From selleckchem.com


A NOVEL METHOD FOR CONTINUOUS DETERMINATION OF THE …
Web A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria. cFSE can be taken up by bacteria in the form of its diacetate …
From ncbi.nlm.nih.gov


EFFECTS OF ATRAZINE ON THE PROLIFERATION AND
Web Feb 1, 2015 Carboxyfluorescein succinimidyl ester (CFDA-SE) has been used as a cell tracker for detecting lymphocyte proliferation (Lyons et al, 2001, Oku et al, 2008).
From sciencedirect.com


CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER - AN OVERVIEW
Web Derek Parsonage, ... Leslie B. Poole, in Methods in Enzymology, 2010 5.2 Chemical modification agents Carboxyfluorescein succinimidyl ester (FAM-SE; 5- and 6-isomers) from Molecular Probes (now Invitrogen, Carlsbad, CA), 473.39 g/mol, dissolved in DMSO
From sciencedirect.com


THE USE OF CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL …
Web Oct 12, 2010 Carboxyfluorescein succinimidyl ester (CFSE) is an effective and popular means to monitor lymphocyte division 1-3. CFSE covalently labels long-lived intracellular molecules with the fluorescent dye, carboxyfluorescein.
From ncbi.nlm.nih.gov


CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER - AN OVERVIEW
Web 4 Labeling OT-1 splenocytes with carboxyfluorescein succinimidyl ester. 1. Prepare 10 mM stock solution of carboxyfluorescein succinimidyl ester (CFSE, Invitrogen, CA) by adding 9 μL DMSO to a vial of Cell Trace CFSE staining solution. Then dilute the stock …
From sciencedirect.com


THERMO SCIENTIFIC™ NHS-FLUORESCEIN (5/6-CARBOXYFLUORESCEIN …
Web Food and Beverage Testing; Glass Fiber Filters and Prefilters; ... Thermo Scientific™ NHS-Fluorescein (5/6-carboxyfluorescein succinimidyl ester), mixed isomer ... Active Ester, Succinimidyl Ester, NHS Ester: Label Type: Classic Dyes: Emission: 518: Quantity: …
From fishersci.co.uk


CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER - AN OVERVIEW ...
Web Studies using propidium iodide or carboxyfluorescein diacetate succinimidyl ester (CFSE) have been used for many years to study cell cycle kinetics of neural precursors [44], but more sophisticated analyses are beginning to be performed.
From sciencedirect.com


A COMPARATIVE STUDY OF CARBOXYFLUORESCEIN DIACETATE AND ...
Web Mar 1, 2003 Carboxyfluorescein diacetate (CFDA) is an esterified fluorogenic substrate that has been widely used for assessing esterase activity in bacteria. CFDA is cell permeant and undergoes hydrolysis of the diacetate (DA) groups into fluorescent …
From sciencedirect.com


NHS-FLUORESCEIN (5/6-CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER), …
Web NHS-Fluorescein is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and …
From thermofisher.com


USING CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER TO MONITOR ...
Web Jun 1, 2015 Therefore, we have used the cell-permeant carboxyfluorescein diacetate linked to a succinimidyl ester moiety (CFDA–SE) to evaluate the extent of cell glycation. Briefly, this assay is based on the specific reactivity of the succinimidyl ester group of …
From sciencedirect.com


CARBOXYFLUORESCEIN | SIGMA-ALDRICH - MILLIPORESIGMA
Web 5-Carboxyfluorescein N-succinimidyl ester. Empirical Formula (Hill Notation): C 25 H 15 NO 9. CAS No.: 92557-80-7. Molecular Weight: 473.39. Beilstein No.: 7865828. Compare Product No. ... -Carboxyfluorescein diacetate N-succinimidyl ester. Synonym(s): …
From sigmaaldrich.com


EFFECTS OF ATRAZINE ON THE PROLIFERATION AND CYTOTOXICITY …
Web In this study, the immunotoxic effects of ATZ on murine splenic lymphocytes and magnetic bead-enriched NK cells were investigated in vitro with the use of carboxyfluorescein succinimidyl ester (CFDA-SE)-based flow cytometric approaches.
From pubmed.ncbi.nlm.nih.gov


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